The MacDonald laboratory seeks a research technician to work with investigators and other laboratory staff to perform laboratory-based research aimed at using cell culture systems, most especially human patient derived induced pluripotent stem cells (iPSC) and their derivatives, to delineate the cell biological effects of mutations that cause or influence the progression of inherited brain disorders (Huntington’s disease, Neuronal Ceroid Lipofuscinosis and Parkinson Disease). The research specifically involves lines of human patient derived iPSC and neuronal cells and other cell types generated from them.
The MacDonald lab maintains, creates and does genetic studies with these patient derived iPSC with the goal of characterizing the genetic basis of these brain diseases, in order to translate these discoveries to improvements in clinical care and public health. We are seeking a highly motivated individual to join our team of talented and intellectually diverse researchers in a collaborative research environment.
PRINCIPAL DUTIES AND RESPONSIBILITIES:
Independently conduct experiments with human patient derived iPSC.
- Grow and maintain stocks of patient derived iPSC and maintain tracking records
- Use cutting edge methods (e.g. CRISPR/Cas9, Zfn or other) to create new human iPSC with targeted mutations in disease genes or modifier genes
- Use different inductive protocols to generate specific cell types (e.g. neuronal cells) from iPSC
- Grow and maintain stocks of iPSC-derivative cell types that are progenitor cells (e.g. neuronal progenitor cell populations)
- Perform standard molecular biological techniques, including PCR, DNA extraction, genotyping, Southern blotting and qPCR.
- Run quality control assays and other sample preparation techniques on iPSC (NPC etc.) in preparation of downstream genetic analysis.
- Perform high content image-based assays with iPSC and derivative cell types (e.g. cell painting)
- Analyze results of high content assays, maintain image data files and extracted features.
- Contribute to troubleshooting experiments and developing new laboratory processes and technologies relevant to iPSC generation, characterization and analysis.
- Attend and participate in lab meetings, including preparing reports and presenting progress.
- Assist in preparation of manuscripts, poster presentations, and project reports.
- Assist in preparation and submission of regulatory materials (e.g. human IRB protocols related to working with patient cells).
- Work to streamline and improve lab processes related to iPSC-based research.
- Maintain log of laboratory inventory, supplies, equipment, and computers involved human cell culture studies.
SKILLS & COMPETENCIES REQUIRED:
- Proficiency in growing, maintaining, characterizing human iPSC or ESC.
- Proficiency in generating human iPSC or derivative cell types (e.g. NPC, neurons).
- Proficiency in molecular biology techniques and previous experience with PCR, genotyping, and/or DNA extraction a plus.
- Proficiency in cell biological techniques and previous experience with high content imaging a plus
- Ability to handle a variety of tasks amid shifting priorities.
- Strong analytical skills with a high degree of initiative.
- Creative and highly motivated individual with strong organizational and management skills.
- Excellent written and verbal communication skills.