Research Fellow (ACTA2) – Kleinstiver Lab – (3188351)

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The Kleinstiver Laboratory ( in the Center for Genomic Medicine at the Massachusetts General Hospital, Harvard Medical School, is seeking highly motivated candidates for a Post-doctoral Fellow position. The recruit for this specific position will play a critical role in managing a collaborative effort dedicated to translating novel genome editing technologies into therapeutics for human diseases. Specifically, in collaboration with Dr. Patricia Musolino (MGH) and Dr. Mark Lindsay (MGH), the candidate will lead the optimization and engineering of safe and efficacious editing approaches to treat pediatric vascular diseases (e.g. multisystem smooth muscle dysfunction syndrome). The applicant will be mentored in all aspects of this research program, providing them the opportunity to play a major role in the pre-clinical development of a new therapeutic approach to treat diseases with major unmet medical needs..


We are recruiting Ph.D.-level scientists to work collaboratively on various projects seeking to bridge the development and optimization of CRISPR genome editing technologies with translational editing approaches. The candidate will play an integral role in projects related to genome editing technology development, optimization, and translation. The Kleinstiver Laboratory develops and applies CRISPR genome editing technologies for biomedical research and the treatment of human diseases. We utilize protein engineering methods to impart desirable properties into CRISPR nucleases, base editors, and prime editors, and seek to translate novel and optimized reagents into molecular medicines. The new hire will be appointed as a Research Fellow at the Massachusetts General Hospital and Post-Doctoral Research Fellow at the Harvard Medical School.


Interested applicants should contact Dr. Ben Kleinstiver ( directly with:

  • a CV detailing scientific experience, technical skills, publications, presentations, examples of mentorship, etc.
  • a brief description of research interests and career goals
  • a summary of their most significant accomplishments




The Post-Doctoral Fellow will become proficient in the following duties (examples, but not limited to): routine molecular biology (PCR, recombinant DNA cloning, bacterial transformations, etc.), biochemical techniques, mammalian cell culture, assessment of genome editing technologies in bacteria and mammalian cells, DNA sequencing methods (Sanger, Illumina next-generation sequencing, Oxford Nanopore, etc.), designing and executing experiments, analyzing and visualizing data, in silico design of molecular constructs, developing and optimizing genome editing assays, attending seminars and conferences, interacting with collaborators and peers, mentoring others in the laboratory, assisting with routine laboratory tasks, preparing reagents and solutions, recording experimental details and results in an electronic notebook, time management, communication skills, and presenting summaries of work or scientific papers in lab meeting and journal club, respectively.


The Post-Doctoral fellow will be situated in the MGH under the guidance and mentorship of Dr. Kleinstiver.



The ideal candidate should have received (or expect to receive soon) a Ph.D. in Biology, Biochemistry, Genetics, Genomics or other closely related fields, or an M.D. Example skills and competencies that the candidate should/could be familiar with include, but are not limited to:

  • Evidence of a strong publication record, including first (or co-first) author of one or more peer-reviewed scientific publications
  • Excellent English verbal and written communication skills
  • Strong proficiency in standard molecular biology techniques
  • Familiarity with genome, epigenetic, and base editing technologies
  • Experience with mammalian cell culture, including transformed cancer cell lines and primary cells
  • Familiarity with Illumina sequencing methods and data analysis
  • A general interest in the scientific literature and staying informed of new advances
  • Able to work both independently and in a team
  • Experience with commonly used bioinformatics tools and databases, and other standard software
  • Familiarity with metabolic and/or neurological diseases
  • Experience mentoring and training others
  • Exceptional organizational skills



  • Doctoral Degree
  • Field of Study/Additional Specialized Training:   Not Applicable



  • Required: Recent PhD graduate




SUPERVISORY RESPONSIBILITY (authority to hire, promote, or terminate):  Not Applicable




WORKING CONDITIONS: work is performed in a laboratory

 EEO Statement 

Massachusetts General Hospital is an Equal Opportunity Employer.  By embracing diverse skills, perspectives and ideas, we choose to lead. Applications from protected veterans and individuals with disabilities are strongly encouraged.
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