GENERAL SUMMARY/ OVERVIEW STATEMENT:
We are seeking an experienced Research Scientist to lead an array of collaborative studies to perform CRISPR-based neuronal modeling of genetic alterations associated with autism spectrum disorder (ASD) and more broadly defined neurodevelopmental disorders (NDDs). The Talkowski Laboratory in the Center for Genomic Medicine at Massachusetts General Hospital (MGH), and the Stanley Center for Psychiatric Research at the Broad Institute of MIT and Harvard, is seeking a motivated, enthusiastic, experienced leader to join our research teams. Our group explores the impact of genomic variation on human disease, with a particular focus on the relationship between genome structure and function in neurodevelopmental and neuropsychiatric disorders. We are also dedicated to the development and application of innovations in genome sequencing technologies and genetic diagnostics. The candidate will be expected to have experience, or high capacity to learn, mammalian cell culture, induced pluripotent stem cell (iPSC) modeling, CRISPR/Cas9 genome editing, and various molecular genetic techniques. The team will include the Principle Investigator and multiple junior faculty, PhD level Staff Scientists, postdoctoral fellows, research technicians and technologists, as well as graduate students. In this position we are seeking a Research Scientist with experience to lead projects, contribute to diverse and highly collaborative research programs, and provide training and oversight to junior scientists.
Highly motivated individuals that may not meet the below qualifications should also see a companion posting (Job ID# 3185183) for research technologist positions within our programs.
PRINCIPAL DUTIES AND RESPONSIBILITIES:
- Perform iPSC studies and neuronal differentiation according to established protocols.
- Perform CRISPR-based genome editing using established protocols
- Learn organoid differentiation across varying lineages, help to drive forward established and novel protocols with team leadership
- Perform molecular genomics procedures as needed (i.e., DNA/RNA extraction, library production, RNAseq, sample archiving, tissue culture, etc.)
- Design, execute, and interpret research experiments
- Learn any number of molecular genomics techniques including single-cell RNAseq (scRNAseq), ATACseq, ChIPseq, HiC, and other methods
- Routinely check medium, reagents, and plasticwares for cell culture experiments
- Maintain laboratory notebook
- Independently follow detailed protocols while working closely with a wide range of lab members (i.e., graduate students, postdocs, technicians, staff scientists)
- Assist in training new Research Technicians and Fellows
- Responsible for troubleshooting problems and instructing others in highly specialized techniques.
- Investigate, create, and develop new methods and technologies for research advancements.
- Preparation of written data reports, including methodology, figure preparations, written description of the study in the form of manuscripts and/or progress reports.
- Contribute to research presentations at local, national, and international meetings.
- Oversight of laboratory technicians participating on the projects, including training them, and assisting them with experimental design and implementation.
- Compose and present sections of research reports.
- Organize and/or draft material for the preparation of research papers, manuscripts, or other documents for publication and/or presentation.
- Ability to work independently and as a team member
- Experience in molecular genomic techniques (i.e., PCR, qPCR, RNAseq, gel electrophoresis)
- Experience in mammalian cell culture required (iPSC culture highly preferred)
- Interest in learning and troubleshooting new molecular biology techniques
- Excellent organizational and communication skills
- Sound interpersonal skills and the ability to supervise and train staff effectively.
- Ability to prioritize tasks, set deadlines, and adapt to shifting priorities in response to changing deadlines and needs of the lab.
- Ability to function with speed, precision, and an understanding of theory behind protocols and experiments.
Ph.D. in biological field required, exceptional candidates with an MS degree in a related field and 5+ years of experience will be considered.
3+ years of experience in a research-related field required, additional experience as a postdoc preferred.
SUPERVISORY RESPONSIBILITY (if applicable):
May oversee research Assistants, Technicians, Technologists, and Students.
Work will be performed in the Simches Research Building on the Massachusetts General Hospital main campus, in Boston, MA.
EEO Statement Massachusetts General Hospital is an Equal Opportunity Employer. By embracing diverse skills, perspectives and ideas, we choose to lead. Applications from protected veterans and individuals with disabilities are strongly encouraged.