The Talkowski Laboratory in the Center for Genomic Medicine at Massachusetts General Hospital (MGH) is seeking a motivated, enthusiastic, experienced candidate to join our research group. Our research group explores the impact of genomic variation on human disease, with a particular focus on neurodevelopment and neuropsychiatric disorders. We are also dedicated to the development and application of innovations in genome sequencing technologies and genetic diagnostics. The candidate will be expected to have extensive experience in the following techniques: DNA/RNA extraction, PCR/qPCR, gel electrophoresis, iPSC culture, mammalian cell culture, aseptic technique, and CRISPR-Cas9 genome editing.
PRINCIPAL DUTIES AND RESPONSIBILITIES
Ensure proper supplies are always stocked
Ensures all necessary solutions are properly prepared and stocked
Ensures all lab reagents, chemicals, instruments and equipment are stocked and maintained
Conducting Experimental Protocols
Must be comfortable working with biological specimens (e.g., tissue, blood, cells) from a variety of specimens (e.g., human, mouse, invertebrates)
Work independently to determine most suitable methodology and protocols for experiments are being performed
Work independently to research and develop modifications of existing assay techniques, experimental protocols, or new procedures
Performs advanced design and modification of protocols
Perform appropriate iPSC culture maintenance and differentiation
Ensures proper execution of standard and developmental protocols
Responsible for troubleshooting problems and instructing others in highly specialized techniques
Work independently to develop new methods based on state-of-the art techniques
Design standardized protocols that can be performed routinely with reproducible results
Maintains laboratory notebook
Conducts analysis of results, and may begin interpretation of results
Calculates, transcribes and analyzes data
Prepares and presents reports
Organizes and summarizes acquired data, using scientific and statistical techniques
Participation in Publication
Perform independent literature searches
Prepares written and/or verbal status reports
Organizing materials and analyze data for publication or presentation
Drafts material for the preparation of research presentations, manuscripts and grants
Laboratory Training and Leadership
Orients and trains new technicians, students and fellows
Train technicians, students and fellows on newly developed protocols and techniques
Supervise multiple technicians and ensure quality of their work
Coordinate with project drivers and analysts to ensure proper integration of data and workflow
Ability to work independently and as a team member
Experience in molecular genomic techniques (i.e. PCR, qPCR, RNAseq, gel electrophoresis)
Experience in mammalian cell culture required (iPSC culture highly preferred)
Interest in learning and troubleshooting new molecular biology techniques
Excellent organizational and communication skills
Sound interpersonal skills and the ability to supervise and train staff effectively.
Ability to prioritize tasks, set deadlines, and adapt to shifting priorities in response to changing deadlines and needs of the lab.
Ability to function with speed, precision and an understanding of theory behind protocols and experiments.
Ph.D. in biological field required
At least 5 years of post-doctoral experience required
May supervise 1-2 Laboratory Assistants, Technicians, and/or Technologists.
Work will be performed in the Simches Research Building on the Massachusetts General Hospital main campus, in Boston, MA.
Massachusetts General Hospital is an Equal Opportunity Employer. By embracing diverse skills, perspectives and ideas, we choose to lead. Applications from protected veterans and individuals with disabilities are strongly encouraged.