Research Fellow – “Gene Therapy for NF1” | Walker Lab – (3231712)

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This is an exciting opportunity for a highly motivated and talented Postdoctoral Research Fellow to join our enthusiastic diverse team to develop cutting-edge gene editing therapies for neurofibromatosis type 1 (NF1). The Walker Laboratory ( focuses primarily on NF1, a genetic disorder with an incidence of one in 3,000–4,000 people. Most adults with NF1 develop neurofibromas – benign, but often disfiguring, tumors associated with peripheral nerves. About 10% of NF1 patients develop malignant peripheral nerve sheath tumors (MPNSTs), which carry a poor prognosis and are often fatal. We utilize a combination of genetic, molecular, and biochemical approaches using both animal and human cell models to improve our mechanistic understanding of NF1 and identify therapeutic targets.


The Postdoctoral Fellow will play an integral role in projects related to developing CRISPR-based genome editing therapeutics (including base and prime editing, as well as CRISPR activation) for individuals with NF1. The project will use molecular biology, biochemical and cell culture techniques, for the design, optimization and testing of genome editing technologies in human cell lines, including iPSCs. The position will involve collaborations with other labs at MGH, Harvard Medical School, and other institutions.



  • Conducting routine molecular biology (PCR, recombinant DNA cloning, bacterial transformations, etc.)
  • Mammalian cell culture – including immortalized tumor cell lines and differentiation of induced pluripotent stem cells (iPSCs)
  • Design, optimization, and testing of CRISPR-based genome editing technologies in mammalian cells
  • DNA sequencing methods (Sanger and Illumina next-generation sequencing)
  • Designing and executing experiments; analyzing, interpreting, and visualizing data
  • Creating and maintaining electronic lab records and inventories
  • Coordinating research activities with collaborators
  • Participating in lab meetings, journal clubs and attend seminars
  • Presenting research findings in scientific papers and at conferences
  • The position offers opportunities to co-mentor Research Technicians, graduate students, and student interns



  • The ideal candidate should have received (or expect to receive soon) a Ph.D. in in a relevant subject (Biology, Biochemistry, Genetics, Genomics or other closely related fields)
  • Example skills and competencies that the candidate should/could be familiar with include, but are not limited to:
  • Strong proficiency in standard molecular biology techniques
  • Experience with mammalian cell culture
  • Familiarity with next generation sequencing methods and data analysis
  • Experience with commonly used bioinformatics tools and databases
  • Excellent English verbal and written communication skills
  • Exceptional organizational skills
  • Proven ability to work independently as well as part of a team in a fast-paced, highly collaborative, and supportive environment
  • Ability to adapt to shifting priorities in response to changing deadlines and the needs of the lab
  • Evidence of a strong publication record, including first (or co-first) author of one or more peer-reviewed scientific publications
  • A general interest in the scientific literature and staying informed of new advances
  • Experience mentoring and training others



  • Doctoral Degree



  • Required: Recent PhD graduate



The information contained in this document is intended to describe the general contents and requirements of work being performed by people assigned to this classification.  It is not intended to be construed as an exhaustive statement of all duties, responsibilities or skills of individuals so classified.

 EEO Statement 

Massachusetts General Hospital is an Equal Opportunity Employer.  By embracing diverse skills, perspectives and ideas, we choose to lead. Applications from protected veterans and individuals with disabilities are strongly encouraged.
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