GENERAL SUMMARY/ OVERVIEW STATEMENT:
Dr. Catarina Silva in the Chemical Neurobiology Lab (CNL) and Dr. Elisabetta Morini in the Center for Genomic Medicine at Massachusetts General Hospital (MGH) are seeking a highly motivated, enthusiastic post-doctoral fellow candidate. Their research will be focused on investigating the role of splicing alterations in the onset of neurodevelopmental and neurodegenerative diseases.
The ideal candidate would have strong attention to detail and excellent time management, organizational, and communication skills. S/he will also have a background and/or interest in neuroscience, medicinal chemistry, and/or human genetics. Tissue culture experience is highly preferred. The research will have a strong emphasis on human iPSC (stem cells) and neuronal cell culture together with biochemical and genetic assays.
The new hire will be appointed as a Research Fellow at the Massachusetts General Hospital, Center for Genomic Medicine, and Post-Doc at the Harvard Medical School.
PRINCIPAL DUTIES AND RESPONSIBILITIES
The Research Fellow will be leading a collaborative research project between Drs. Morini and Silva’s labs aimed at understanding the role of splicing alterations in neurological diseases with the objective of developing novel mRNA-targeted therapeutics.
He/ She will generate CRISPR-engineered human induced pluripotent stem cell (iPSC)-derived neurons for several disease-causing splicing variants to evaluate the impact of aberrant splicing on neuronal phenotypes. Interest in computational biology is also highly desirable.
The Research Fellow will also be responsible for project management, data analysis, preparing presentation materials, manuscripts, and grant applications, mentoring technologists and students, and presentations at local, national, and international conferences.
The Post-Doctoral fellow will be situated at MGH under the guidance and mentorship of Drs. Morini and Silva.
SKILLS & COMPETENCIES REQUIRED:
The ideal candidate should have received (or expect to receive soon) a Ph.D. in neuroscience, cell or molecular biology, human genetics, biochemistry, or related field.
Desired skills and competencies:
- Molecular biology techniques including DNA, RNA, and protein isolation and manipulation (RT-PCR, qRT-PCR, western blotting)
- Good understanding of genetic methodologies, with experience in cloning, CRISPR/Cas genetic editing techniques, genomic sequencing
- Some level of cell culture experience, preferably with iPSC/stem cells and/or neuronal cell culture, to include cell lines generation, transfection/transduction, selection, propagation, preservation, and phenotypic characterization
- Some experience with microscopy imaging and immuno-cytochemistry (IHC) techniques
- Histological and immune-histochemical techniques.
- First (or co-first) author of one or more peer-reviewed scientific publications
- Excellent English verbal and written communication skills
- Able to work both independently and in a tea
LICENSES, CERTIFICATIONS, and/or REGISTRATIONS:
Required: Recent PhD graduate/Soon to be PhD
SUPERVISORY RESPONSIBILITY (authority to hire, promote, or terminate):